Recombinant Human CD33 Protein, Fc/His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: CD33-833HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human CD33, transcript variant 1, fused with Fc/His tag at C-terminal was expressed in HEK293 cells.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc/His
• Form: Lyophilized
• Molecular Mass: 55.01 kDa
• AA Sequence: DPNFWLQVQESVTVQEGLCVLVPCT FFHPIPYYDKNSPVHGYWFREGAII SGDSPVATNKLDQEVQEETQGRFRL LGDPSRNNCSLSIVDARRRDNGSYF FRMERGSTKYSYKSPQLSVHVTDLT HRPKILIPGTLEPGHSKNLTCSVSW ACEQGTPPIFSWLSAAPTSLGPRTT HSSVLIITPRPQDHGTNLTCQVKFA GAGVTTERTIQLNVTYVPQNPTTGI FPGDGSGKQETRAGVVHTSDIEGRM DEPKSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWL NGKEYKCKVSNKALPAPIEKTISKA KGQPREPQVYTLPPSREEMTKNQVS LTCLVKGFYPSDIAVEWESNGQPEN NYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQK SLSLSPGKVDHHHHHH
• Endotoxin: < 0.1 ng/ μg of protein (< 1 EU/ μg).
• Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, 2 mM EDTA, pH 7.2
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens ]
• Official Symbol: CD33
• Synonyms: CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3
• Gene ID: 945
• mRNA Refseq: NM_001082618
• Protein Refseq: NP_001076087
• MIM: 159590
• UniProt ID: P20138