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Recombinant Human CD33 Protein, LIgG2b Fc-tagged, low endotoxin

Cat.No.: CD33-342H
Product Overview: Recombinant Human CD33 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1). Predicted N-terminus: Asp 18. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 54.6 kDa. The protein migrates as 64-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source: HEK293
Species: Human
Tag : LIgG2b Fc
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Recombinant Human CD33 protein, LIgG2b Fc-tagged, low endotoxin at 5 μg/mL (100 μL/well) can bind Anti-CD33, Human IgG1 with a linear range of 0.078-1.25 μg/mL (QC tested).
Molecular Mass: 54.6 kDa
Protein length: Asp 18 - His 259
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: CD33 CD33 molecule [ Homo sapiens ]
Official Symbol: CD33
Synonyms: CD33; CD33 molecule; CD33 antigen (gp67); FLJ00391; p67; SIGLEC 3; SIGLEC3; gp67; SIGLEC-3;
Gene ID: 945
mRNA Refseq: NM_001082618
Protein Refseq: NP_001076087
MIM: 159590
UniProt ID: P20138

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