Active Recombinant Human CD40 protein, His/Fc-tagged, Biotinylated
Cat.No. : | CD40-202H |
Product Overview : | Recombinant Human CD40(Leu 25 - Ser 645) fused with His and human IgG1 Fc fragment tag at C-terminal was expressed in HEK293, was enzymatically single-point biotinlabeled at C-terminus to retain its native conformational structure and bioactivity. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His/Fc |
Predicted N Terminal : | Glu 21 |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Ligand, His Tag at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human CD40, His Tag & Fc Tag with a linear range of 0.2-1μg/mL. |
Molecular Mass : | Has a calculated MW of 48 kDa. DTT-reduced Protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation. |
Endotoxin : | Less than 1.0 EU per μg of the Biotinylated Human CD40, His Tag & Fc tag by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Applications : | Has been used successfully for ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes. |
Storage : | Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade). |
Gene Name : | CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ] |
Official Symbol : | CD40 |
Synonyms : | CD40; CD40 molecule, TNF receptor superfamily member 5; TNFRSF5, tumor necrosis factor receptor superfamily, member 5; tumor necrosis factor receptor superfamily member 5; Bp50; p50; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule; CDW40; TNFRSF5; MGC9013; |
Gene ID : | 958 |
mRNA Refseq : | NM_001250 |
Protein Refseq : | NP_001241 |
MIM : | 109535 |
UniProt ID : | P25942 |
Chromosome Location : | 20q12-q13.2 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; |
Function : | enzyme binding; protein binding; receptor activity; signal transducer activity; |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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