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Active Recombinant Human CD70 protein, hFc-tagged

Cat.No. : CD70-639
Product Overview : Recombinant Human CD70 protein(NP_001243.1)(Gln39-Pro193), fused with hFc tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : hFc
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human CD27 His at 2 μg/ml (100 μl/well) can bind Human CD70 hFc, the EC50 of Human CD70 hFc is 15-60 ng/mL.
Molecular Mass : The recombinant human CD70/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 413 amino acids and predicts a molecular mass of 45.5 kDa. As a result of different glycosylation, the rh CD70/Fc migrates as several bands with the approximate molecular mass of 55-60, 110-120 and 160-170 kDa corresponding to the monomeric, dimeric and trimeric form respectively in SDS-PAGE under reducing conditions.
Proteinlength : Gln39-Pro193
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : CD70 CD70 molecule [ Homo sapiens ]
Official Symbol : CD70
Synonyms : CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7;
Gene ID : 970
mRNA Refseq : NM_001252
Protein Refseq : NP_001243
MIM : 602840
UniProt ID : P32970

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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