Recombinant Human CDK2AP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CDK2AP1-5275H
• Product Overview: CDK2AP1 MS Standard C13 and N15-labeled recombinant protein (NP_004633) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 12.4 kDa
• AA Sequence: MSYKPNLAAHMPAAALNAAGSVHSP STSMATSSQYRQLLSDYGPPSLGYT QGTGNSQVPQSKYAELLAIIEELGK EIRPTYAGSKSAMERLKRGIIHARG LVRECLAETERNARSTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CDK2AP1 cyclin-dependent kinase 2 associated protein 1 [ Homo sapiens (human) ]
• Official Symbol: CDK2AP1
• Synonyms: CDK2AP1; cyclin-dependent kinase 2 associated protein 1; CDK2 associated protein 1; cyclin-dependent kinase 2-associated protein 1; doc 1; DOC1; DORC1; p12DOC 1; ST19; Deleted in oral cancer-1; deleted in oral cancer 1; CDK2-associated protein 1; putative oral cancer suppressor; doc-1; p12DOC-1
• Gene ID: 8099
• mRNA Refseq: NM_004642
• Protein Refseq: NP_004633
• MIM: 602198
• UniProt ID: O14519