Recombinant Human CRBN Protein (1-442 aa), His-tagged

• Cat.No.: CRBN-2793H
• Product Overview: Recombinant Human CRBN Protein (1-442 aa) is produced by Baculovirus expression system. This protein is fused with a 9xHis tag at the C-terminal. Research Area: Neuroscience. Protein Description: Full Length.

Specification

• Description: Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
• Source: Baculovirus
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 52.5 kDa
• Protein length: 1-442 aa
• AA Sequence: MAGEGDQQDAAHNMGNHLPLLPAES EEEDEMEVEDQDSKEAKKPNIINFD TSLPTSHTYLGADMEEFHGRTLHDD DSCQVIPVLPQVMMILIPGQTLPLQ LFHPQEVSMVRNLIQKDRTFAVLAY SNVQEREAQFGTTAEIYAYREEQDF GIEIVKVKAIGRQRFKVLELRTQSD GIQQAKVQILPECVLPSTMSAVQLE SLNKCQIFPSKPVSREDQCSYKWWQ KYQKRKFHCANLTSWPRWLYSLYDA ETLMDRIKKQLREWDENLKDDSLPS NPIDFSYRVAACLPIDDVLRIQLLK IGSAIQRLRCELDIMNKCTSLCCKQ CQETEITTKNEIFSLSLCGPMAAYV NPHGYVHETLTVYKACNLNLIGRPS TEHSWFPGYAWTVAQCKICASHIGW KFTATKKDMSPQKFWGLTRSALLPT IPDTEDEISPDKVILCL
• Purity: > 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: CRBN cereblon [ Homo sapiens ]
• Official Symbol: CRBN
• Synonyms: CRBN; cereblon; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715
• Gene ID: 51185
• mRNA Refseq: NM_001173482
• Protein Refseq: NP_001166953
• MIM: 609262
• UniProt ID: Q96SW2