Recombinant Human CTLA4 protein, Twin Strep-tagged CTLA4-2235H

Recombinant Human CTLA4 protein, Twin Strep-tagged

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Recombinant Human CTLA4 protein, Twin Strep-tagged

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Cat.No. : CTLA4-2235H
Product Overview : Recombinant Human CTLA4(Ala 37 - Phe 162) fused with Twin Strep tag at C-terminal was expressed in HEK293.
Source : HEK293
Species : Human
Tag : Twin Strep
Predicted N Terminal : Ala 37
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD80 /B7-1 Protein, With C-Fc Tag at 2 μg/mL ( 100 μl/well ) can bind Recombinant Human CTLA4 /CD152 Protein, With C-Twin Strep Tag with a linear range of 1-20 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-2 / CD86 Protein, With C-Fc Tag at 2 μg/mL ( 100 μl/well ) can bind Recombinant Human CTLA4 /CD152 Protein, With C-Twin Strep Tag with a linear range of 1-20 ng/mL.
Molecular Mass : Has a calculated MW of 16.5 kDa. DTT-reduced Protein migrates as 21-26 kDa in SDS-PAGE due to glycosylation.
Protein length : Ala 37 - Phe 162
Endotoxin : Less than 1.0 EU per μg of the rh CTLA4 /CD152,twin strep tag by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
Gene Name : CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]
Official Symbol : CTLA4
Synonyms : CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3;
Gene ID : 1493
mRNA Refseq : NM_005214
Protein Refseq : NP_005205
MIM : 123890
UniProt ID : P16410
Chromosome Location : 2q33
Pathway : Adaptive Immune System, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CTLA4 inhibitory signaling, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem;
Function : protein binding;

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