Active Recombinant Human CYP17A1 Protein

• Cat.No.: CYP17A1-87H
• Product Overview: Recombinant human CYP17A1 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
• Source: E. coli
• Species: Human
• Bio-activity: Cytochrome c Reductase Activity: 68 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Progesterone 17α-hydroxylase
  Linear with time up to: 20 min(Determined at 1.2 µM substrate, using a P450 concentration of 10 pmol/ml)
  Linear with P450 up to: 20 pmol/ml(Determined at 1.2 µM substrate, using an incubation time of 5 min)
  Vmax: 2.0 pmol/min/pmol CYP
  Km: 0.11 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 2.6 nmol/ml
  Protein Concentration: 11.8 mg/ml
  Specific P450 Content: 220 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP17A1 cytochrome P450 family 17 subfamily A member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP17A1
• Synonyms: CYP17A1; cytochrome P450 family 17 subfamily A member 1; CPT7; CYP17; S17AH; P450C17; steroid 17-alpha-hydroxylase/17,20 lyase; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; cytochrome P450 17A1; cytochrome P450, family 17, subfamily A, polypeptide 1; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome P450-C17; cytochrome P450c17; cytochrome p450 XVIIA1; steroid 17-alpha-monooxygenase
• Gene ID: 1586
• mRNA Refseq: NM_000102
• Protein Refseq: NP_000093
• MIM: 609300
• UniProt ID: P05093