Active Recombinant Human CYP1A1 Protein

• Cat.No.: CYP1A1-04H
• Product Overview: Recombinant Human CYP1A1 and human CYP-low reductase are coexpressed in Escherichia coli.

Specification

• Description: This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
• Source: E.coli
• Species: Human
• Form: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
• Bio-activity: Specific P450 Content:100 pmol/mg protein; Cytochrome c Reductase Activity:103 nmol/min/mg protein; P450 Activity:7-ethoxycoumarin O-deethylase; Linear with time up to:12 min; Linear with P450 up to:5 pmol/ml; Vmax:11 pmol/min/pmol CYP; Km:3.8 μM
• Notes: 1. Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]; 2. Determined at 5 μM substrate, using a P450 concentration of 1 pmol/ml; 3. Determined at 5 μM substrate, using an incubation time of 5 min.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: 10 mg/ml

Gene Information

• Gene Name: CYP1A1
• Official Symbol: CYP1A1
• Synonyms: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• Gene ID: 1543
• mRNA Refseq: NM_000499.5
• Protein Refseq: NP_000490.1
• MIM: 108330
• UniProt ID: P04798