Active Recombinant Human CYP1A2 Protein

• Cat.No.: CYP1A2-01H
• Product Overview: Recombinant Human CYP1A2 and human CYP-reductase are coexpressed in Escherichia coli.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
• Source: E.coli
• Species: Human
• Form: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
• Bio-activity: Specific P450 Content:100 pmol/mg protein; Cytochrome c Reductase Activity:26 nmol/min/mg protein; P450 Activity: 7-ethoxycoumarin O-deethylase; Linear with time up to:15 min; Linear with P450 up to:10 pmol/ml; Vmax:0.56 pmol/min/pmol CYP; Km:2.6 μM.
• Notes: 1.Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]. 2.Determined at 2 μM substrate, using a P450 concentration of 2.5 pmol/ml. 3.Determined at 2 μM substrate, using an incubation time of 5 min.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: 10 mg/ml

Gene Information

• Gene Name: CYP1A2
• Official Symbol: CYP1A2
• Synonyms: CP12; CYPIA2; P3-450; P450(PA)
• Gene ID: 1544
• mRNA Refseq: NM_000761.5
• Protein Refseq: NP_000752.2
• MIM: 124060
• UniProt ID: P05177