Active Recombinant Human CYP2E1 Protein

• Cat.No.: CYP2E1-221H
• Product Overview: Human CYP2E1 and human CYP-reductase coexpressed in Escherichia coli, supplemented with purified human cytochrome b5.

Specification

• Source: E.coli
• Species: Human
• Bio-activity: Cytochrome c Reductase Activity: 300 nmol/min/mg protein
  #1 P450 Activity Data
  Activity: Chlorzoxazone 6-hydroxylase
  #2 Linear with time up to: 15 min
  #3 Linear with P450 up to: 40 pmol/ml
  Vmax: 19 pmol/min/pmol CYP
  Km: 165 µM
  Notes:
  #1: Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase].
  #2: Determined at 120 µM substrate, using a P450 concentration of 25 pmol/ml.
  #3: Determined at 120 µM substrate, using an incubation time of 5 min.
• Residual Host Cell Protein Content_: Specific P450 Content: 100 pmol/mg protein
• Notes: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Cytochrome b5 Concentration: 5.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
• Warning: The toxicological properties of this reagent have not been investigated. Exercise due care when handling.

Gene Information

• Gene Name: CYP2E1 cytochrome P450 family 2 subfamily E member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP2E1
• Synonyms: CYP2E1; cytochrome P450 family 2 subfamily E member 1; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; EC 1.14.13.n7; EC 1.14.14.1
• Gene ID: 1571
• mRNA Refseq: NM_000773
• Protein Refseq: NP_000764
• MIM: 124040
• UniProt ID: P05181