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Recombinant Human cytomegalovirus GB protein, Fc-tagged

Cat.No.: GB-18H
Product Overview: Recombinant Human cytomegalovirus GB(Val 23 - Lys 700) fused with a human IgG1 Fc tag at the C-terminus, with furin cleavage site mutated from 'RTKR' to 'TTQT' was expressed in HEK293.
Source: HEK293
Species: Human cytomegalovirus
Tag: Fc
Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Molecular Mass: Has a calculated MW of 104 kDa. The predicted N-terminus is Val 23. DTT-reduced Protein migrates as 140-150 kDa in SDS-PAGE due to glycosylation.
Endotoxin: Less than 1.0 EU per μg of the rh CMV-GB Fc Chimera by the LAL method.
Purity: >85% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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