Description : |
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Fc/His |
Form : |
Lyophilized from a 0.2 µM filtered solution of 20mM PB, 150mM NaCl, pH7.2 |
Molecular Mass : |
58.9 kDa |
AA Sequence : |
EPKSCDKTHTCPPCPAPELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTK PREEQYNSTYRVVSVLTVLHQDWLN GKEYKCKVSNKALPAPIEKTISKAK GQPREPQVYTLPPSREEMTKNQVSL TCLVKGFYPSDIAVEWESNGQPENN YKTTPPVLDSDGSFFLYSKLTVDKS RWQQGNVFSCSVMHEALHNHYTQKS LSLSPGKVDGGGSGGGSGGGSRKRR MAALMRSKDSSCCLLLLAAVLMVES SQIGSSRAKLNSIKSSLGGETPGQA ANRSAGMYQGLAFGGSKKGKNLGQA YPCSSDKECEVGRYCHSPHQGSSAC MVCRRKKKRCHRDGMCCPSTRCNNG ICIPVTESILTPHIPALDGTQHRDR NHGHYSNHDLGWQNLGRPHTKMSHI KGHEGDPCLRSSDCIEGFCCARHFW TKICKPVLHQGEVCTKQRKKGSHGL EIFQRCDCAKGLSCKVWKDATYSSK ARLHVCQKIVDHHHHHH |
Endotoxin : |
Endotoxin level is <0.1 ng/µg of protein (<1EU/µg). |
Purity : |
>95% as determined by SDS-PAGE and Coomassie blue staining |
Concentration : |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |