Active Recombinant Human EGF Protein

• Cat.No.: EGF-021H
• Product Overview: The mature recombinant human EGF protein containing 80aa (N971-R1023) without tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Source: E. coli
• Species: Human
• Bio-activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg.
• Molecular Mass: 6 kDa
• Product-Related Proteins: 971-1023
• Endotoxin: < 0.1 EU/μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: At -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: PBS

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens (human) ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; URG; HOMG4; pro-epidermal growth factor; beta-urogastrone
• Gene ID: 1950
• mRNA Refseq: NM_001963
• Protein Refseq: NP_001954
• MIM: 131530
• UniProt ID: P01133