Recombinant Human EGLN2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EGLN2-4013H
• Product Overview: EGLN2 MS Standard C13 and N15-labeled recombinant protein (NP_444274) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 43.7 kDa
• AA Sequence: MDSPCQPQPLSQALPQLPGSSSEPL EPEPGRARMGVESYLPCPLLPSYHC PGVPSEASAGSGTPRATATSTTASP LRDGFGGQDGGELRPLQSEGAAALV TKGCQRLAAQGARPEAPKRKWAEDG GDAPSPSKRPWARQENQEAEREGGM SCSCSSGSGEASAGLMEEALPSAPE RLALDYIVPCMRYYGICVKDSFLGA ALGGRVLAEVEALKRGGRLRDGQLV SQRAIPPRSIRGDQIAWVEGHEPGC RSIGALMAHVDAVIRHCAGRLGSYV INGRTKAMVACYPGNGLGYVRHVDN PHGDGRCITCIYYLNQNWDVKVHGG LLQIFPEGRPVVANIEPLFDRLLIF WSDRRNPHEVKPAYATRYAITVWYF DAKERAAAKDKYQLASGQKGVQVPV SQPPTPTTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EGLN2 egl-9 family hypoxia inducible factor 2 [ Homo sapiens (human) ]
• Official Symbol: EGLN2
• Synonyms: EGLN2; egl nine homolog 2 (C. elegans); EGL nine (C.elegans) homolog 2; egl nine homolog 2; HIF prolyl hydroxylase 1; HIFPH1; PHD1; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 1; prolyl hydroxylase domain-containing protein 1; EIT6; HPH-1; HPH-3; HIF-PH1; FLJ95603; DKFZp434E026
• Gene ID: 112398
• mRNA Refseq: NM_053046
• Protein Refseq: NP_444274
• MIM: 606424
• UniProt ID: Q96KS0