Recombinant Human EIF3D Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EIF3D-896H
• Product Overview: EIF3D MS Standard C13 and N15-labeled recombinant protein (NP_003744) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 64 kDa
• AA Sequence: MAKFMTPVIQDNPSGWGPCAVPEQF RDMPYQPFSKGDRLGKVADWTGATY QDKRYTNKYSSQFGGGSQYAYFHEE DESSFQLVDTARTQKTAYQRNRMRF AQRNLRRDKDRRNMLQFNLQILPKS AKQKERERIRLQKKFQKQFGVRQKW DQKSQKPRDSSVEVRSDWEVKEEMD FPQLMKMRYLEVSEPQDIECCGALE YYDKAFDRITTRSEKPLRSIKRIFH TVTTTDDPVIRKLAKTQGNVFATDA ILATLMSCTRSVYSWDIVVQRVGSK LFFDKRDNSDFDLLTVSETANEPPQ DEGNSFNSPRNLAMEATYINHNFSQ QCLRMGKERYNFPNPNPFVEDDMDK NEIASVAYRYRRWKLGDDIDLIVRC EHDGVMTGANGEVSFINIKTLNEWD SRHCNGVDWRQKLDSQRGAVIATEL KNNSYKLARWTCCALLAGSEYLKLG YVSRYHVKDSSRHVILGTQQFKPNE FASQINLSVENAWGILRCVIDICMK LEEGKYLILKDPNKQVIRVYSLPDG TFSSDEDEEEEEEEEEEEEEEETTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EIF3D eukaryotic translation initiation factor 3 subunit D [ Homo sapiens (human) ]
• Official Symbol: EIF3D
• Synonyms: EIF3D; eukaryotic translation initiation factor 3, subunit D; EIF3S7, eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; eukaryotic translation initiation factor 3 subunit D; eIF3 p66; eIF3 zeta; eIF3d; eIF-3-zeta; translation initiation factor eIF3 p66 subunit; eukaryotic translation initiation factor 3 subunit 7; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; EIF3S7; eIF3-p66; eIF3-zeta; MGC17258; MGC126526
• Gene ID: 8664
• mRNA Refseq: NM_003753
• Protein Refseq: NP_003744
• MIM: 603915
• UniProt ID: O15371