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Recombinant Human EIF4A2 Protein, Myc/DDK-tagged, C13 and N15-labeled

Cat.No. : EIF4A2-4364H
Product Overview : EIF4A2 MS Standard C13 and N15-labeled recombinant protein (NP_001958) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
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Description : ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 46.4 kDa
AA Sequence : MSGGSADYNREHGGPEGMDPDGVIE SSWNEIVDNFDDMNLKESLLRGIYA YGFEKPSAIQQRAIIPCIKGYDVIA QAQSGTGKTATFAISILQQLEIEFK ETQALVLAPTRELAQQIQKVILALG DYMGATCHACIGGTNVRNEMQKLQA EAPHIVVGTPGRVFDMLNRRYLSPK WIKMFVLDEADEMLSRGFKDQIYEI FQKLNTSIQVVLLSATMPTDVLEVT KKFMRDPIRILVKKEELTLEGIKQF YINVEREEWKLDTLCDLYETLTITQ AVIFLNTRRKVDWLTEKMHARDFTV SALHGDMDQKERDVIMREFRSGSSR VLITTDLLARGIDVQQVSLVINYDL PTNRENYIHRIGRGGRFGRKGVAIN FVTEEDKRILRDIETFYNTTVEEMP MNVADLITRTRPLEQKLISEEDLAA NDILDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
Gene Name : EIF4A2 eukaryotic translation initiation factor 4A2 [ Homo sapiens (human) ]
Official Symbol : EIF4A2
Synonyms : EIF4A2; eukaryotic translation initiation factor 4A2; EIF4F, eukaryotic translation initiation factor 4A, isoform 2; eukaryotic initiation factor 4A-II; BM 010; DDX2B; EIF4A; ATP-dependent RNA helicase eIF4A-2; eukaryotic translation initiation factor 4A, isoform 2; EIF4F; BM-010; eIF4A-II; eIF-4A-II;
Gene ID : 1974
mRNA Refseq : NM_001967
Protein Refseq : NP_001958
MIM : 601102
UniProt ID : Q14240

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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