| Description : |
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
175.3 kDa |
| AA Sequence : |
MNKAPQSTGPPPAPSPGLPQPAFPP GQTAPVVFSTPQATQMNTPSQPRQH FYPSRAQPPSSAASRVQSAAPARPG PAAHVYPAGSQVMMIPSQISYPASQ GAYYIPGQGRSTYVVPTQQYPVQPG APGFYPGASPTEFGTYAGAYYPAQG VQQFPTGVAPAPVLMNQPPQIAPKR ERKTIRIRDPNQGGKDITEEIMSGA RTASTPTPPQTGGGLEPQANGETPQ VAVIVRPDDRSQGAIIADRPGLPGP EHSPSESQPSSPSPTPSPSPVLEPG SEPNLAVLSIPGDTMTTIQMSVEES TPISRETGEPYRLSPEPTPLAEPIL EVEVTLSKPVPESEFSSSPLQAPTP LASHTVEIHEPNGMVPSEDLEPEVE SSPELAPPPACPSESPVPIAPTAQP EELLNGAPSPPAVDLSPVSEPEEQA KEVTASMAPPTIPSATPATAPSATS PAQEEEMEEEEEEEEGEAGEAGEAE SEKGGEELLPPESTPIPANLSQNLE AAAATQVAVSVPKRRRKIKELNKKE AVGDLLDAFKEANPAVPEVENQPPA GSNPGPESEGSGVPPRPEEADETWD SKEDKIHNAENIQPGEQKYEYKSDQ WKPLNLEEKKRYDREFLLGFQFIFA SMQKPEGLPHISDVVLDKANKTPLR PLDPTRLQGINCGPDFTPSFANLGR TTLSTRGPPRGGPGGELPRGPAGLG PRRSQQGPRKEPRKIIATVLMTEDI KLNKAEKAWKPSSKRTAADKDRGEE DADGSKTQDLFRRVRSILNKLTPQM FQQLMKQVTQLAIDTEERLKGVIDL IFEKAISEPNFSVAYANMCRCLMAL KVPTTEKPTVTVNFRKLLLNRCQKE FEKDKDDDEVFEKKQKEMDEAATAE ERGRLKEELEEARDIARRRSLGNIK FIGELFKLKMLTEAIMHDCVVKLLK NHDEESLECLCRLLTTIGKDLDFEK AKPRMDQYFNQMEKIIKEKKTSSRI RFMLQDVLDLRGSNWVPRRGDQGPK TIDQIHKEAEMEEHREHIKVQQLMA KGSDKRRGGPPGPPISRGLPLVDDG GWNTVPISKGSRPIDTSRLTKITKP GSIDSNNQLFAPGGRLSWGKGSSGG SGAKPSDAASEAARPATSTLNRFSA LQQAVPTESTDNRRVVQRSSLSRER GEKAGDRGDRLERSERGGDRGDRLD RARTPATKRSFSKEVEERSRERPSQ PEGLRKAASLTEDRDRGRDAVKREA ALPPVSPLKAALSEEELEKKSKAII EEYLHLNDMKEAVQCVQELASPSLL FIFVRHGVESTLERSAIAREHMGQL LHQLLCAGHLSTAQYYQGLYEILEL AEDMEIDIPHVWLYLAELVTPILQE GGVPMGELFREITKPLRPLGKAASL LLEILGLLCKSMGPKKVGTLWREAG LSWKEFLPEGQDIGAFVAEQKVEYT LGEESEAPGQRALPSEELNRQLEKL LKEGSSNQRVFDWIEANLSEQQIVS NTLVRALMTAVCYSAIIFETPLRVD VAVLKARAKLLQKYLCDEQKELQAL YALQALVVTLEQPPNLLRMFFDALY DEDVVKEDAFYSWESSKDPAEQQGK GVALKSVTAFFKWLREAEEESDHNT RTRPLEQKLISEEDLAANDILDYKD DDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
