Recombinant Human EIF5 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EIF5-3395H
• Product Overview: EIF5 MS Standard C13 and N15-labeled recombinant protein (NP_001960) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 49.2 kDa
• AA Sequence: MSVNVNRSVSDQFYRYKMPRLIAKV EGKGNGIKTVIVNMVDVAKALNRPP TYPTKYFGCELGAQTQFDVKNDRYI VNGSHEANKLQDMLDGFIKKFVLCP ECENPETDLHVNPKKQTIGNSCKAC GYRGMLDTHHKLCTFILKNPPENSD SGTGKKEKEKKNRKGKDKENGSVSS SETPPPPPPPNEINPPPHTMEEEED DDWGEDTTEEAQRRRMDEISDHAKV LTLSDDLERTIEERVNILFDFVKKK KEEGVIDSSDKEIVAEAERLDVKAM GPLVLTEVLFNEKIREQIKKYRRHF LRFCHNNKKAKRYLLHGLECVVAMH QAQLISKIPHILKEMYDADLLEEEV IISWSEKASKKYVSKELAKEIRVKA EPFIKWLKEAEEESSGGEEEDEDEN IEVVYSKAASVPKVETVKSDNKDDD IDIDAITRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EIF5 eukaryotic translation initiation factor 5 [ Homo sapiens (human) ]
• Official Symbol: EIF5
• Synonyms: EIF5; eukaryotic translation initiation factor 5; eIF-5; EIF-5A
• Gene ID: 1983
• mRNA Refseq: NM_001969
• Protein Refseq: NP_001960
• MIM: 601710
• UniProt ID: P55010