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Recombinant Human FCGR2A Protein, His-tagged, Biotinylated

Cat.No.: FCGR2A-2965H
Product Overview: Biotinylated Human Fc gamma RIIA / CD32a (R131) (CDA-H82E7) is expressed from human HEK293 cells. It contains AA Ala 35 - Ile 217 (Accession # AAH19931).
Source: HEK293
Species: Human
Tag: His
Predicted N Terminal: Ala 35
Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (1μg/well). The linear range is 0.15-10 μg/mL.
Molecular Mass: This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24 kDa. The protein migrates as 30-38 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
Official Symbol: FCGR2A
Synonyms: FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032;
Gene ID: 2212
mRNA Refseq: NM_001136219
Protein Refseq: NP_001129691
MIM: 146790
UniProt ID: P12318
Chromosome Location: 1q23
Pathway: Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Leishmaniasis, organism-specific biosystem; Leishmaniasis, conserved biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Phagosome, organism-specific biosystem;
Function: IgG binding; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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