Active Recombinant Human FCGRT & B2M Heterodimer Protein, His & Avi & Strep II-tagged, Biotinylated
Cat.No. : | FCGRT & B2M-377H |
Product Overview : | Recombinant Human FCGRT & B2M Heterodimer Protein, His & Avi & Strep II-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)). Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M). Biotinylated Human FcRn Heterodimer Protein (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His & Avi & Strep II |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Recombinant Human FCGRT & B2M Heterodimer Protein, His & Avi & Strep II-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested). |
Molecular Mass : | 34.1 kDa (FCGRT) & 13.1 kDa (B2M) |
Protein length : | Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by reduced SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ] & B2M beta-2-microglobulin [ Homo sapiens ] |
Official Symbol : | FCGRT & B2M |
Synonyms : | FCGRT; alpha chain; FCRN; FcRn alpha chain; alpha-chain; & B2M; beta-2-microglobulin; |
Gene ID : | 2217 & 567 |
mRNA Refseq : | NM_001136019 & NM_004048 |
Protein Refseq : | NP_001129491 & NP_004039 |
MIM : | 601437 & 109700 |
UniProt ID : | P55899 & P61769 |
Products Types
◆ Recombinant Protein | ||
FCGRT & B2M-1050H | Recombinant Human FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged | +Inquiry |
FCGRT & B2M-379F | Active Recombinant Feline FCGRT & B2M Heterodimer Protein, His & Avi-tagged, Biotinylated | +Inquiry |
FCGRT & B2M-378B | Active Recombinant Bovine FCGRT & B2M Heterodimer Protein, His & Avi-tagged, Biotinylated | +Inquiry |
FCGRT & B2M-1150C | Recombinant Cynomolgus FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged, Biotinylated | +Inquiry |
FCGRT & B2M-103CB | Recombinant Cynomolgus FCGRT & B2M protein, His-Avi-tagged, Biotinylated | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket