Active Recombinant Human FCGRT/B2M Protein, His & Strep & Avi-tagged, Biotinylated
Cat.No. : | FCGRT & B2M-1926H |
Product Overview : | Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein (FCM-H82W4) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FcRN)(Accession # NP_001129491.1 & Ile 21 - Met 119 (B2M) (Accession # AAH32589). |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His & Strep & Avi |
Predicted N Terminal : | Ala 24(FcRN) & Ile 21(B2M) |
Form : | Lyophilized from sterile PBS, pH7.4, 10% Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (Human IgG1, 1μg/well). The linear range is 0.3-5 μg/mL. Biotinylated Human FcRn / FCGRT & B2M, Avi Tag coupled to SA coated sensor chip can bind Herceptin with an affinity constant of 2.2-2.6 μM as determined in a SPR assay (Biacore 2000). |
Molecular Mass : | The subunit FCGRT / FcRN carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus. The protein has a calculated MW of 33 kDa (FcRN), 13.1 kDa (B2M). The protein migrates as 36 kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing condition due to glycosylation. |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ] |
Official Symbol : | FCGRT |
Synonyms : | FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain; |
Gene ID : | 2217 |
mRNA Refseq : | NM_001136019 |
Protein Refseq : | NP_001129491 |
MIM : | 601437 |
UniProt ID : | P55899 |
Chromosome Location : | 19q13.3 |
Function : | IgG binding; beta-2-microglobulin binding; receptor activity; |
Products Types
◆ Recombinant Protein | ||
FCGRT & B2M-1249M | Active Recombinant Mouse FCGRT & B2M Protein, His-tagged | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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