Active Recombinant Human FGFR1 Protein, His/GST-tagged, Alexa Fluor 488 conjugated

• Cat.No.: FGFR1-236HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect cells
• Species: Human
• Tag: His/GST
• Bio-activity: 1. Kinase activity untested.
  2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) at 10 μg/mL (100 μL/well) can bind biotinylated human FGF acidic with a linear range of 15.6-250 ng/mL.
  3. Measured by i
• Molecular Mass: The recombinant human FGFR1 (aa 311-731) /GST chimera consists of 658 amino acids and has a calculated molecular mass of 75 kDa. It migrates as an approximately 75 kDa band as predicted in SDS-PAGE under reducing conditions.
• Protein length: 658
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 93 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 20% glycerol, 0.3 mM DTT.

Gene Information

• Gene Name: FGFR1 fibroblast growth factor receptor 1 [ Homo sapiens ]
• Official Symbol: FGFR1
• Synonyms: FGFR1; fibroblast growth factor receptor 1; FLT2, fms related tyrosine kinase 2 , KAL2; BFGFR; CD331; CEK; FLG; H2; H3; H4; H5; N SAM; Pfeiffer syndrome; FGFR1/PLAG1 fusion; proto-oncogene c-Fgr; FMS-like tyrosine kinase 2; hydroxyaryl-protein kinase; fms-related tyrosine kinase 2; heparin-binding growth factor receptor; basic fibroblast growth factor receptor 1; OGD; FLT2; KAL2; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1; FLJ99988
• Gene ID: 2260
• mRNA Refseq: NM_001174063
• Protein Refseq: NP_001167534
• MIM: 136350
• UniProt ID: P11362