| Description : |
GALNT1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the GalNAc-T family of enzymes. It utilizes UDP-GalNAc as the nucleotide donor substrate to modify serine or threonine residues of proteins trafficking through the secretory pathway. Structurally, GalNAc-Ts consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant human GALNT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Source : |
Insect cell |
| Species : |
Human |
| Tag : |
His |
| Form : |
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10 % glycerol. |
| Bio-activity : |
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that transfer 1.0 pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute per minute at pH 8.0 at 37 centigrade. |
| Molecular Mass : |
60.4 kDa |
| Protein length : |
41-559 aa |
| AA Sequence : |
ADPGLPAGDV LEPVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSKGQVITF LDAHCECTVG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRVGLRHKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCNGSRSQQ WLLRNVTLPE IFHHHHHH |
| Endotoxin : |
< 1.0 EU per 1 ug of protein (determined by LAL method) |
| Purity : |
> 90 % by SDS - PAGE |
| Notes : |
For research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
| Storage : |
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
1 mg/ml (determined by Absorbance at 280 nm). |
