"GLA" Related Products

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Recombinant Human GLA protein, His-tagged

Cat.No.: GLA-45H
Product Overview: Recombinant Human GLA(Met1-Leu429) fused with His tag at C-terminal was expressed in CHO.
Source: CHO
Species: Human
Tag : His
Predicted N Terminal: Leu32
Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Bio-activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-galactopyranoside. The specific activity is >1,100 pmol/min/μg.
Molecular Mass: Predicted Molecular Mass: 46 kDa;
SDS-PAGE: 45-55 kDa, reducing conditions.
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Storage: Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Gene Name: GLA galactosidase, alpha [ Homo sapiens ]
Official Symbol: GLA
Synonyms: GLA; galactosidase, alpha; alpha-galactosidase A; GALA; melibiase; alpha-gal A; agalsidase alfa; alpha-D-galactosidase A; alpha-D-galactoside galactohydrolase 1;
Gene ID: 2717
mRNA Refseq: NM_000169
Protein Refseq: NP_000160
MIM: 300644
UniProt ID: P06280
Chromosome Location: Xq21.3-q22
Pathway: Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycosphingolipid biosynthesis - globo series, organism-specific biosystem; Glycosphingolipid biosynthesis - globo series, conserved biosystem; Glycosphingolipid metabolism, organism-specific biosystem;
Function: alpha-galactosidase activity; alpha-galactosidase activity; alpha-galactosidase activity; catalytic activity; cation binding; galactoside binding; hydrolase activity; hydrolase activity, hydrolyzing O-glycosyl compounds; protein binding; protein homodimerization activity; raffinose alpha-galactosidase activity; receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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