Recombinant Human GOT1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GOT1-1495H
• Product Overview: GOT1 MS Standard C13 and N15-labeled recombinant protein (NP_002070) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.2 kDa
• AA Sequence: MAPPSVFAEVPQAQPVLVFKLTADF REDPDPRKVNLGVGAYRTDDCHPWV LPVVKKVEQKIANDNSLNHEYLPIL GLAEFRSCASRLALGDDSPALKEKR VGGVQSLGGTGALRIGADFLARWYN GTNNKNTPVYVSSPTWENHNAVFSA AGFKDIRSYRYWDAEKRGLDLQGFL NDLENAPEFSIVVLHACAHNPTGID PTPEQWKQIASVMKHRFLFPFFDSA YQGFASGNLERDAWAIRYFVSEGFE FFCAQSFSKNFGLYNERVGNLTVVG KEPESILQVLSQMEKIVRITWSNPP AQGARIVASTLSNPELFEEWTGNVK TMADRILTMRSELRARLEALKTPGT WNHITDQIGMFSFTGLNPKQVEYLV NEKHIYLLPSGRINVSGLTTKNLDY VATSIHEAVTKIQTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GOT1 glutamic-oxaloacetic transaminase 1 [ Homo sapiens (human) ]
• Official Symbol: GOT1
• Synonyms: GOT1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); aspartate aminotransferase, cytoplasmic; transaminase A; growth-inhibiting protein 18; glutamate oxaloacetate transaminase 1; GIG18; ASTQTL1
• Gene ID: 2805
• mRNA Refseq: NM_002079
• Protein Refseq: NP_002070
• MIM: 138180
• UniProt ID: P17174