| Description : |
This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
80.8 kDa |
| AA Sequence : |
MSLFLSNLSTNDSSLWKENHNSTDL LNPPGTLNIYLFCLTCLMTFAALVG SIYSLISLLKMQNRTVVSMLVASWS VDDLMSVLSVTIFMFLQWPNEVPGY FQFLCTTSALMYLCQGLSSNLKATL LVSYNFYTMHRGVGSQTASRRSGQV LGVVLTVWAASLLLSALPLCGWGAF VRTPWGCLVDCSSSYVLFLSIVYAL AFGLLVGLSVPLTHRLLCSEEPPRL HSNYQEISRGASIPGTPPTAGRVVS LSPEDAPGPSLRRSGGCSPSSDTVF GPGAPAAAGAEACRRENRGTLYGTR SFTVSVAQKRFALILALTKVVLWLP MMMHMVVQNVVGFQSLPLETFSFLL TLLATTVTPVFVLSKRWTHLPCGCI INCRQNAYAVASDGKKIKRKGFEFN LSFQKSYGIYKIAHEDYYDDDENSI FYHNLMNSECETTKDPQRDNRNIFN AIKVEISTTPSLDSSTQRGINKCTN TDITEAKQDSNNKKDAFSDKTGGDI NYEETTFSEGPERRLSHEESQKPDL SDWEWCRSKSERTPRQRSGYALAIP LCAFQGTVSLHAPTGKTLSLSTYEV SAEGQKITPASKKIEVYRSKSVGHE PNSEDSSSTFVDTSVKIHLEVLEIC DNEEALDTVSIISNISQSSTQVRSP SLRYSRKENRFVSCDLGETASYSLF LPTSNPDGDINISIPDTVEAHRQNS KRQHQERDGYQEEIQLLNKAYRKRE EESKGSTRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
