Recombinant Human GTF2F1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GTF2F1-665H
• Product Overview: GTF2F1 MS Standard C13 and N15-labeled recombinant protein (NP_002087) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 58.2 kDa
• AA Sequence: MAALGPSSQNVTEYVVRVPKNTTKK YNIMAFNAADKVNFATWNQARLERD LSNKKIYQEEEMPESGAGSEFNRKL REEARRKKYGIVLKEFRPEDQPWLL RVNGKSGRKFKGIKKGGVTENTSYY IFTQCPDGAFEAFPVHNWYNFTPLA RHRTLTAEEAEEEWERRNKVLNHFS IMQQRRLKDQDQDEDEEEKEKRGRR KASELRIHDLEDDLEMSSDASDASG EEGGRVPKAKKKAPLAKGGRKKKKK KGSDDEAFEDSDDGDFEGQEVDYMS DGSSSSQEEPESKAKAPQQEEGPKG VDEQSDSSEESEEEKPPEEDKEEEE EKKAPTPQEKKRRKDSSEESDSSEE SDIDSEASSALFMAKKKTPPKRERK PSGGSSRGNSRPGTPSAEGGSTSST LRAAASKLEQGKRVSEMPAAKRLRL DTGPQSLSGKSTPQPPSGKTTPNSG DVQVTEDAVRRYLTRKPMTTKDLLK KFQTKKTGLSSEQTVNVLAQILKRL NPERKMINDKMHFSLKETRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GTF2F1 general transcription factor IIF subunit 1 [ Homo sapiens (human) ]
• Official Symbol: GTF2F1
• Synonyms: GTF2F1; general transcription factor IIF, polypeptide 1, 74kDa; general transcription factor IIF, polypeptide 1 (74kD subunit); general transcription factor IIF subunit 1; BTF4; RAP74; TF2F1; TFIIF; TFIIF-alpha; transcription initiation factor RAP74; general transcription factor IIF 74 kDa subunit; transcription initiation factor IIF subunit alpha
• Gene ID: 2962
• mRNA Refseq: NM_002096
• Protein Refseq: NP_002087
• MIM: 189968
• UniProt ID: P35269