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Active Recombinant Human GZMB protein, His-tagged

Cat.No. : GZMB-315H
Product Overview : Recombinant Human GZMB(Gly19-Tyr247) fused with His tag at C-terminal was expressed in NS0.
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  • Gene Information
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Description : Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections. It activates various caspases and cleaves proteins such as aggrecan. Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247). The rhGranzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously.
Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Gly19
Form : Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >1,000 pmol/min/µg
Molecular Mass : Predicted Molecular Mass: 27 kDa;SDS-PAGE: 37 kDa, reducing conditions
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.
Gene Name : GZMB granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) [ Homo sapiens ]
Official Symbol : GZMB
Synonyms : GZMB; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); CSPB, CTLA1; granzyme B; cathepsin G like 1; CCPI; CGL 1; CGL1; CSP B; CTSGL1; cytotoxic serine protease B; fragmentin 2; HLP; SECT; T cell serine protease 1 3E; C11; CTLA-1; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic T-lymphocyte proteinase 2; CSPB; CGL-1; CSP-B; CTLA1;
Gene ID : 3002
mRNA Refseq : NM_004131
Protein Refseq : NP_004122
MIM : 123910
UniProt ID : P10144
Chromosome Location : 14q11.2
Pathway : Activation, myristolyation of BID and translocation to mitochondria, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem;
Function : catalytic activity; peptidase activity; protein binding; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are some potential future applications of GZMB in medicine? 02/05/2020

GZMB may be used in the development of targeted therapies for viral infections and various immune-related disorders.

How is GZMB protein clinically relevant in cancer treatment? 07/28/2018

GZMB can be used in cancer immunotherapy to target and eliminate cancer cells.

What are the challenges in using GZMB in cancer immunotherapy? 09/14/2016

One challenge is to ensure that GZMB selectively targets cancer cells without causing harm to healthy cells.

What types of cancer can be targeted using GZMB-based therapies? 07/24/2016

GZMB-based therapies can be used against various types of cancer, including leukemia, lymphoma, and solid tumors.

Can GZMB be used in autoimmune disease treatment? 03/22/2016

GZMB's role in autoimmune diseases is complex. It can contribute to tissue damage in some autoimmune conditions, but its use in treatment is not common.

Customer Reviews (3)

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Reviews
11/01/2020

    I am delighted to express my complete satisfaction with the quality of GZMB protein, as it precisely suits my experimental requirements.

    08/29/2017

      Their knowledgeable team has been readily available to address any concerns or queries, providing effective solutions and guidance whenever needed.

      07/28/2017

        I have full confidence in the GZMB protein and the exceptional support provided by the manufacturer, making it my top recommendation for researchers seeking a high-quality protein for their experimental needs.

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