Active Recombinant Human GZMB protein, His-tagged
Cat.No. : | GZMB-315H |
Product Overview : | Recombinant Human GZMB(Gly19-Tyr247) fused with His tag at C-terminal was expressed in NS0. |
- Specification
- Gene Information
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Description : | Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections. It activates various caspases and cleaves proteins such as aggrecan. Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247). The rhGranzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously. |
Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Gly19 |
Form : | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
Bio-activity : | Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >1,000 pmol/min/µg |
Molecular Mass : | Predicted Molecular Mass: 27 kDa;SDS-PAGE: 37 kDa, reducing conditions |
Endotoxin : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening. |
Gene Name : | GZMB granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) [ Homo sapiens ] |
Official Symbol : | GZMB |
Synonyms : | GZMB; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); CSPB, CTLA1; granzyme B; cathepsin G like 1; CCPI; CGL 1; CGL1; CSP B; CTSGL1; cytotoxic serine protease B; fragmentin 2; HLP; SECT; T cell serine protease 1 3E; C11; CTLA-1; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic T-lymphocyte proteinase 2; CSPB; CGL-1; CSP-B; CTLA1; |
Gene ID : | 3002 |
mRNA Refseq : | NM_004131 |
Protein Refseq : | NP_004122 |
MIM : | 123910 |
UniProt ID : | P10144 |
Chromosome Location : | 14q11.2 |
Pathway : | Activation, myristolyation of BID and translocation to mitochondria, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; |
Function : | catalytic activity; peptidase activity; protein binding; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type peptidase activity; |
Products Types
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◆ Lysates | ||
GZMB-2944HCL | Recombinant Human GZMB cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0403 | GZMB Activity Fluorometric Assay Kit | +Inquiry |
Kit-0404 | GZMB Assay/Inhibitor Screening Fluorometric Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionGZMB may be used in the development of targeted therapies for viral infections and various immune-related disorders.
GZMB can be used in cancer immunotherapy to target and eliminate cancer cells.
One challenge is to ensure that GZMB selectively targets cancer cells without causing harm to healthy cells.
GZMB-based therapies can be used against various types of cancer, including leukemia, lymphoma, and solid tumors.
GZMB's role in autoimmune diseases is complex. It can contribute to tissue damage in some autoimmune conditions, but its use in treatment is not common.
Customer Reviews (3)
Write a reviewI am delighted to express my complete satisfaction with the quality of GZMB protein, as it precisely suits my experimental requirements.
Their knowledgeable team has been readily available to address any concerns or queries, providing effective solutions and guidance whenever needed.
I have full confidence in the GZMB protein and the exceptional support provided by the manufacturer, making it my top recommendation for researchers seeking a high-quality protein for their experimental needs.
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