Recombinant Human HAO1 Protein, His-tagged

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Recombinant Human HAO1 Protein, His-tagged

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Cat.No. : HAO1-001H
Product Overview : Recombinant human HAO1 protein(1-370aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description : HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones.
Source : E. coli
Species : Human
Tag : His
Form : Liquid
Bio-activity : Specific activity is > 3,000 pmol/min/μg, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25 centigrade.
Molecular Mass : 45.0 kDa
AA Sequence : MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI
Purity : > 95% by SDS-PAGE
Notes : For research use only. This product is not intended or approved for human, diagnostics or veterin.
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
Avoid repeated freezing and thawing cycles.
Concentration : 1.0 mg/mL
Storage Buffer : 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.5M NaCl.
Gene Name : HAO1 hydroxyacid oxidase 1 [ Homo sapiens (human) ]
Official Symbol : HAO1
Synonyms : GOX (glycolate oxidase); GOX1; HAOX1; Hydroxyacid oxidase 1; GOX; hydroxyacid oxidase 1; (S)-2-hydroxy-acid oxidase; glycolate oxidase 1; hydroxyacid oxidase (glycolate oxidase) 1; EC 1.1.3.15
Gene ID : 54363
mRNA Refseq : NM_017545
Protein Refseq : NP_060015
MIM : 605023
UniProt ID : Q9UJM8

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