|Product Overview:||Recombinant Human HAVCR1(Ser21-Thr288) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.|
|Description:||T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses. TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain. Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. Within the extracellular domain, human TIM-1 shares 41% aa sequence identity with mouse and rat TIM-1. TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium. It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury. Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation. TIM-1 serves as a receptor for phosphatidylserine, LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells and iNKT cell activation. TIM-1 binding induces the activation of LMIR5-expressing myeloid cells, contributing to tissue homeostasis as well as damage following kidney injury. TIM-1 ligation co-stimulates T cell activation and enhances Th2 cytokine production. In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus.|
|Predicted N Terminal:||Ser21|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is typically 0.6-3.6 μg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 55 kDa
SDS-PAGE: 112-129 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 500 μg/mL in PBS.|
|Gene Name:||HAVCR1 hepatitis A virus cellular receptor 1 [ Homo sapiens ]|
|Synonyms:||HAVCR1; hepatitis A virus cellular receptor 1; HAVCR; HAVCR 1; TIM 1; TIM1; TIMD1; kidney injury molecule 1; T-cell membrane protein 1; T cell immunoglobin domain and mucin domain protein 1; TIM; KIM1; KIM-1; TIM-1; TIMD-1; HAVCR-1;|
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