Recombinant Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein (tetramer), His-Avi-tagged

• Cat.No.: HLA-A-069H
• Product Overview: Human HLA-A*0201 WT-1 (RMFPNAPYL) complex protein(P01892 /P61769 & RMFPNAPYL) is expressed from mammalian with a polyHis and avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly 25 - Thr 305 (HLA-A*0201),Ile21-Met119 (B2M) and RMFPNAPYL peptide design.

Specification

• Description: The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLAA*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein has a calculated MW of 50.5kDa. The protein (tetramer) migrates as 240-260 kDa under Non reducing (N) condition (Bis-Tris PAGE).
• Protein length: It contains Gly 25 - Thr 305 (HLA-A*0201),Ile21-Met119 (B2M) and RMFPNAPYL peptide design.
• Endotoxin: Less than 0.1EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A; B2M
• Synonyms: MHC; WT1; RMF; Wilms tumor protein; HLA-A; B2M
• Gene ID: 3105
• mRNA Refseq: NM_001242758.1
• Protein Refseq: NP_001229687.1
• UniProt ID: P01891