Recombinant Human HLA-DRB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HLA-DRB1-4573H
• Product Overview: HLA MS Standard C13 and N15-labeled recombinant protein (NP_002115) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and some alleles have increased frequencies associated with certain diseases. There are multiple pseudogenes of this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 29.97 kDa
• AA Sequence: MVCLKLPGGSCMTALTVTLMVLSSP LALAGDTRPRFLWQLKFECHFFNGT ERVRLLERCIYNQEESVRFDSDVGE YRAVTELGRPDAEYWNSQKDLLEQR RAAVDTYCRHNYGVGESFTVQRRVE PKVTVYPSKTQPLQHHNLLVCSVSG FYPGSIEVRWFRNGQEEKAGVVSTG LIQNGDWTFQTLVMLETVPRSGEVY TCQVEHPSVTSPLTVEWRARSESAQ SKMLSGVGGFVLGLLFLGAGLFIYF RNQKGHSGLQPTGFLSTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HLA-DRB1 major histocompatibility complex, class II, DR beta 1 [ Homo sapiens (human) ]
• Official Symbol: HLA-DRB1
• Synonyms: HLA-DRB1; major histocompatibility complex, class II, DR beta 1; HLA DR1B; DW2.2/DR2.2; MHC class II antigen; lymphocyte antigen DRB1; MHC class II HLA-DRw10-beta; human leucocyte antigen DRB1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; HLA class II histocompatibility antigen, DR-1 beta chain; SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B; FLJ75017; FLJ76359
• Gene ID: 3123
• mRNA Refseq: NM_002124
• Protein Refseq: NP_002115
• MIM: 142857
• UniProt ID: P01911