Recombinant Human HSPA1A Protein, His-tagged HSPA1A-015H

Recombinant Human HSPA1A Protein, His-tagged

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Recombinant Human HSPA1A Protein, His-tagged

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Cat.No. : HSPA1A-015H
Product Overview : Recombinant full length human HSPA1A protein (2-641) witha N-terminal Histidine-tag was purified from E. coli
Description : HSPA4 heat shock protein family A (Hsp70) member 4 are abundant and stress-inducible 70 kDa molecular chaperone proteinsencoded by a highly conserved, multigene family. They are monomeric proteins that can be divided into two functional domains: an N-terminal ATPase domain and a substrate binding domain that contains a highly conserved EEVD motif at its C-terminus. Hsp70s are found in the cytosol, nuclei, endoplasmic reticulum, mitochondria, and chloroplasts of eukaryotes, as well as in bacteria. They function as molecular chaperones that assist in a wide range of cellular processes, including refolding of aggregated or misfolded proteins, co- and post-translational folding and assembly of nascent peptides, membrane translocation of secretory and organellar proteins, controlling activity of regulatory nuclear receptors, kinases and transcription factors, as well as acting cooperatively with the Hsp90 chaperone system in eukaryotes. The HSPA4 chaperone cycle is ATP-dependent and initiated by transient interaction of the HSPA4 substrate binding domain with hydrophobic regions within a peptide or protein.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 1 mM DTT, and 10% glycerol
Bio-activity : ATPase activity confirmed by ADP detection assay
Molecular Mass : 71.7 kDa
Protein length : 2-641
Purity : ≥75% estimated by SDS-PAGE
Stability : ≥ 1 year
Storage : Store at -80 centigrade
Shipping : Dry ice in continental US; may vary elsewhere
SDS-PAGE analysis of HSPA4. :

Lane 1: MW Markers
Lane 2: HSPA4 BV (1 µg)
Lane 3: HSPA4 BV (2 µg)
Lane 4: HSPA4 BV (4 µg)

ATPase Activity of HSPA4 Expressed In Insect Cell. :

Gene Name : HSPA1A heat shock protein family A (Hsp70) member 1A [ Homo sapiens (human) ]
Official Symbol : HSPA1A
Synonyms : HSPA1A; heat shock protein family A (Hsp70) member 1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103; heat shock 70 kDa protein 1A; HSP70-1/HSP70-2; HSP70.1/HSP70.2; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein
Gene ID : 3303
mRNA Refseq : NM_005345
Protein Refseq : NP_005336
MIM : 140550
UniProt ID : P0DMV8

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