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Active Recombinant Human IL10 protein

Cat.No. : IL10-29629TH
Product Overview : Recombinant Human IL10 protein(NP_000563.1)(Ser 19-Asn 178) was expressed in E. coli.
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  • Gene Information
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Source : E. coli
Species : Human
Tag : N/A
Form : Lyophilized from sterile 20mM Tris, 20mM NaCl, pH 6.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Immobilized human IL10 at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA-Fc, The EC50 of Cynomolgus IL10RA-Fc is 0.24-0.56 μg/mL.2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells.The ED50 for this effect is typically 0.5-2.5 ng/mL.
Molecular Mass : The recombinant human GADD45G consists of 160 amino acids and predicts a molecular mass of 18.6 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
Protein Length : Ser 19-Asn 178
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : IL10 interleukin 10 [ Homo sapiens ]
Official Symbol : IL10
Synonyms : IL10; interleukin 10; interleukin-10; CSIF; cytokine synthesis inhibitory factor; IL 10; IL10A; T cell growth inhibitory factor; TGIF; T-cell growth inhibitory factor; GVHDS; IL-10; MGC126450; MGC126451;
Gene ID : 3586
mRNA Refseq : NM_000572
Protein Refseq : NP_000563
MIM : 124092
UniProt ID : P22301

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What state-of-the-art experimental strategies, including advanced imaging techniques, are implemented to meticulously assess the in vivo efficacy of IL-10-based therapies across a spectrum of inflammatory disorders? 02/12/2022

Cutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.

How are advanced methods like single-cell RNA sequencing and flow cytometry harnessed to investigate the immunomodulatory impact of IL-10 across distinct immune cell subtypes? 02/10/2022

Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.

Could you elaborate on the intricate experimental methodologies applied to unravel the influence of genetic polymorphisms within IL-10 and its receptor on disease susceptibility? 10/26/2021

Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.

How do researchers leverage advanced immunomonitoring tools to comprehensively evaluate the safety profiles of IL-10-based interventions in various clinical trial phases? 05/25/2021

Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.

How do researchers employ in vitro models to elucidate the mechanisms underlying IL-10's anti-inflammatory effects on immune cells? 12/03/2020

In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.

What sophisticated experimental techniques are utilized to dissect the intricate signaling pathways downstream of IL-10 activation? 08/26/2020

State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.

Could you explain the multifaceted experimental strategies employed to determine the optimal dosage and administration routes for IL-10 in diverse animal models? 03/24/2019

Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.

What innovative experimental designs are employed to explore potential synergies between IL-10 and other cytokines, unraveling complex interplay in immune regulation? 11/23/2018

Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.

Can you elaborate on the comprehensive approaches scientists use to assess the tissue-specific expression patterns of IL-10 receptors in both normal and pathological conditions? 11/05/2018

Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.

How are cutting-edge techniques such as CRISPR/Cas9 gene editing utilized to dissect the regulatory roles of IL-10 in complex autoimmune disease models? 02/22/2018

CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.

Customer Reviews (3)

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Reviews
09/27/2022

    Effective in anti-inflammatory assays.

    01/09/2019

      High purity levels.

      04/15/2017

        Stable under long-term storage.

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