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Active Recombinant Human IL16 protein

Cat.No. : IL16-126H
Product Overview : Recombinant Human IL16 was expressed in E. coli.
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  • Gene Information
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Description : Interleukin 16 (IL16) is a pleiotrophic cytokine derived from CD8+ T-cells that stimulates chemotaxis in CD4+ lymphocytes, monocytes, and eosinophils. It primes CD4+ T-cells for IL2 and IL15 responsiveness, induces T-lymphocyte Interleukin 2 (IL2) receptor expression, and inhibits HIV replication. The signalling process of IL16 is mediated by CD4, and undergoes proteolysis to yield two functional proteins. IL16 isoform 1 has the possible function of acting as a scaffolding protein to anchor ion channels in the membrane, while isoform 3 is involved in cell cycle progression in T-cells and transcriptional regulation of SKP2. IL16 mRNA expression levels have been found in various tissues such as spleen, bone marrow, thymus, and lymph nodes.
Source : E. coli
Species : Human
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : The ED50 is 7.5-37.5 ng/mL as determined by its ability to chemoattract human CD4+ T lymphocytes (at a concentration range of 1.0-100.0 ng/mL).
Molecular Mass : 13.5 kDa
Endotoxin : <1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity : >95%
Applications : Cell Assay
Storage : The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade - 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Reconstitution : Reconstitute in sterile PBS at 5 μg/Ml
Gene Name : IL16 interleukin 16 [ Homo sapiens ]
Official Symbol : IL16
Synonyms : IL16; interleukin 16; interleukin 16 (lymphocyte chemoattractant factor); pro-interleukin-16; FLJ16806; FLJ42735; HsT19289; IL 16; LCF; lymphocyte chemoattractant factor; prIL 16; prointerleukin 16; neuronal interleukin 16; NIL16; PRIL16; prIL-16; FLJ44234;
Gene ID : 3603
mRNA Refseq : NM_001172128
Protein Refseq : NP_001165599
MIM : 603035
UniProt ID : Q14005
Chromosome Location : 15q26.3
Function : cytokine activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How is IL16's chemotactic activity assessed in leukocyte migration assays? 12/10/2022

Leukocyte migration assays measure IL16's chemotactic impact on cells.

What methods investigate IL16's modulation of T cell activation markers? 09/26/2022

Flow cytometry and qPCR assess T cell activation markers affected by IL16.

How do gene expression arrays unveil IL16's role in inflammatory responses? 05/18/2022

Gene expression arrays reveal IL16's influence on inflammatory gene profiles.

Could you detail the use of RNA interference in knocking down IL16 expression? 01/17/2022

RNA interference used to knock down IL16 expression in cell lines.

Can you explain the experimental setup for studying IL16's impact on HIV infection? 07/25/2021

Experimental setup for studying IL16's modulation of HIV infection involves viral assays.

What techniques determine IL16's binding affinity to CD4 receptors? 10/12/2019

Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.

How are flow cytometry and confocal microscopy utilized to examine IL16's localization? 12/06/2018

Flow cytometry and confocal microscopy visualize IL16's cellular localization.

What experimental models aid in deciphering IL16's role in autoimmune disorders? 07/24/2017

Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.

Could you elaborate on the use of ELISA in quantifying IL16 secretion? 05/16/2017

ELISA quantifies secreted IL16 levels from cell culture supernatants.

What strategies discern IL16's involvement in allergic diseases? 01/30/2017

Strategies like animal models and patient samples explore IL16's role in allergies.

Customer Reviews (3)

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Reviews
08/31/2021

    Suitable for ELISA.

    06/28/2019

      High specificity in ELISA.

      02/05/2018

        Potent in chemotaxis studies.

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