Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged
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|Product Overview :||Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)). Two sequential affinity purification steps were used to ensure exact 1:1 molar ratio of IL17A ＆IL17F heterodimer. Predicted N-terminus: His (IL-17A) & Ser (IL-17F). Human IL-17A & IL-17F Heterodimer Protein, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 17.0 kDa (IL-17A) and 18.4 kDa (IL-17F). Subunit IL-17A is fused with a polyhistidine tag at the N-terminus and subunit IL-17F is fused with a Twin Strep tag at the N-terminus. The predicted N-terminus is His (IL-17A) & Ser (IL-17F). The reducing (R) protein migrates as 19 kDa (IL-17A) and 21 kDa (IL-17F) respectively due to glycosylation.|
|Tag :||His & Strep II|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 39-625 ng/mL (QC tested).
Immobilized Anti-IL17A Mab Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged with a linear range of 0.2-3 ng/mL (Routinely tested).
|Molecular Mass :||17.0 kDa (IL-17A) & 18.4 kDa (IL-17F)|
|Protein length :||Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F)|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by reduced SDS-PAGE.|
|Notes :||Please avoid repeated freeze-thaw cycles.|
|Stability :||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name :||IL17A interleukin 17A [ Homo sapiens ] & IL17F interleukin 17F [ Homo sapiens ]|
|Official Symbol :||IL17A & IL17F|
|Synonyms :||IL17A; interleukin 17A; IL 17; IL 17A; CTLA-8; IL17; CTLA8; IL-17; IL-17A; & IL17F; interleukin 17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; ML-1; CANDF6; IL-17F;|
|Gene ID :||3605 & 112744|
|mRNA Refseq :||NM_002190 & NM_052872|
|Protein Refseq :||NP_002181 & NP_443104|
|MIM :||603149 & 606496|
|UniProt ID :||Q16552 & Q96PD4|
◆ Recombinant Protein
|IL17A&IL17F-339H||Recombinant Human IL17A & IL17F Protein, His & Avi-tagged, Biotinylated||+Inquiry|