Active Recombinant Human IL1RL1, His-tagged, Biotinylated

• Cat.No.: IL1RL1-629H
• Product Overview: The recombinant human IL1RL1 ECD is expressed as a 320 amino acid protein consisting of Lys19 - Ser328 region of IL1RL1/ST2 (UniProt accession #Q01638) and a C-terminal His-tag.

Specification

• Source: Human cells
• Species: Human
• Tag: His
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Immobilized IL1RL1 binds human IL33 in a functional ELISA. Blocks IL33-dependent signaling activity in helper T cells.
• Molecular Mass: Calculated molecular mass (kDa): 36.3; Estimated by SDS-PAGE under reducing condition (kDa): 60-70 (probably due to glycosylation).
• AA Sequence: KFSKQSWGLENEALIVRCPRQGKPS YTVDWYYSQTNKSIPTQERNRVFAS GQLLKFLPAAVADSGIYTCIVRSP TFNRTGYANVTIYKKQSDCNVPDYL MYSTVSGSEKNSKIYCPTIDLYNWT APLEWFKNCQALQGSRYRAHKSFL VIDNVMTEDAGDYTCKFIHNENGAN YSVTATRSFTVKDEQGFSLFPVIGA PAQNEIKEVEIGKNANLTCSACFG KGTQFLAAVLWQLNGTKITDFGEPR IQQEEGQNQSFSNGLACLDMVLRIA DVKEEDLLLQYDCLALNLHGLRR HTVRLSRKNPIDHHSTGHHHHHHHH
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the lal>
• Purity: >90% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL1RL1 interleukin 1 receptor-like 1 [ Homo sapiens ]
• Official Symbol: IL1RL1
• Synonyms: IL1RL1; interleukin 1 receptor-like 1; interleukin-1 receptor-like 1; DER4; FIT 1; homolog of mouse growth stimulation expressed; IL33R; ST2; ST2L; ST2V; T1; growth stimulation-expressed; interleukin 1 receptor-related protein; homolog of mouse growth stimulation-expressed; FIT-1; MGC32623
• Gene ID: 9173
• mRNA Refseq: NM_003856
• Protein Refseq: NP_003847
• MIM: 601203
• UniProt ID: Q01638
• Chromosome Location: 2q12
• Function: cytokine receptor activity; interleukin-1 receptor activity; interleukin-33 binding; protein binding; receptor activity; receptor signaling protein activity