Active Recombinant Human IL4, His-tagged
Cat.No. : | IL4-27H |
Product Overview : | Recombinant Human IL4 was produced in the endosperm tissue of barley grain (Hordeum vulgare). |
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Description : | IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells. |
Source : | Barley grain (Hordeum vulgare) |
Species : | Human |
Tag : | His |
Form : | Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH7,2 |
Bio-activity : | Recombinant human IL-4 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically< 0.3 ng/ml, corresponding to a specific activity>3.3 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines. |
Molecular Mass : | Recombinant human IL-4 contains 130 amino acids and a 16 a.a. Histidine-based tag for a total length of 146 a.a. and has a predicted molecular mass of 17.2 kDa. Due to glycosylation the recombinant protein migrates as smeary band with an apparent molecular mass of 23 kDa in SDS-PAGE. |
Endotoxin : | Endotoxin level is less than 0.005ng per μg of product (<> |
Purity : | Greater than 95% by SDS-PAGE gel analysis. |
Storage : | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile low salt buffer to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | IL4 interleukin 4 [ Homo sapiens ] |
Official Symbol : | IL4 |
Synonyms : | IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1; |
Gene ID : | 3565 |
mRNA Refseq : | NM_000589 |
Protein Refseq : | NP_000580 |
MIM : | 147780 |
UniProt ID : | P05112 |
Chromosome Location : | 5q23-q31 |
Pathway : | Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD40/CD40L signaling, organism-specific biosystem; |
Function : | cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewBy testing immunogenicity in animal models, detect whether an immune response is induced.
The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.
This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.
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