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Recombinant Human IL5 protein

Cat.No.: IL5-241H
Product Overview: Recombinant Human IL5 was expressed in Insect cells.
Source: Insect cells
Species: Human
Form: This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Bio-activity: The biological activity of Human IL-5 was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED50 for this effect is typically 0.1 - 0.2 ng/ml.
Molecular Mass: The predicted molecular weight of Recombinant Human IL-5 is Mr 13.1 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 13-15 kDa.
AA Sequence: i pteiptsalv ketlallsth rtllianetl ripvpvhknh qlcteeifqg igtlesqtvq ggtverlfkn lslikkyidg qkkkcgeerr rvnqfldylq eflgvmntew iies
Endotoxin: <1.0 EU/µg as determined by the LAL method
Purity: >97% by SDS-PAGE and analyzed by silver stain.
Storage: Working aliquots of this recombinant protein solution are stable for up to twelve months at -20 centigrade to -70 centigrade in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2 centigrade - 8 centigrade for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
Official Symbol: IL5
Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5;
Gene ID: 3567
mRNA Refseq: NM_000879
Protein Refseq: NP_000870
MIM: 147850
UniProt ID: P05113
Chromosome Location: 5q23-q31
Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem;
Function: cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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