| Description : |
Recombinant human IL-6 is an important pro-inflammatory and anti-inflammatory cytokine expressed by many types cell including: T and B cells, macrophages, endothelial cells, fibroblasts, monocytes, keratinocytes and certain tumor cells. It is a multifunctional cytokine that modulates several physiologic processes such as haematopoiesis, stimulation of immunoglobulin synthesis, maturation and activation of B cells, differentiation of T lymphocytes and regulation of the hepatic acute-phase response. IL-6 is also produced in muscle, is discharged into the bloodstream after muscle contraction and acts increasing the breakdown of fats and improving insulin resistance. IL-6 induces signalling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signalling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signalling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. |
| Source : |
Nicotiana benthamiana |
| Species : |
Human |
| Tag : |
His |
| Form : |
Recombinant human IL-6 is lyophilized from 10mM Phosphate Potasium buffer pH 7.4 and 50mM NaCl. |
| Bio-activity : |
The specific activity is determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erytroleukemic indicator cell line). ED50< 1> |
| Molecular Mass : |
rhuman Interleukin-6 is a polypeptide chain containing 183 amino acids (30 – 212 of P05231 IL6_HUMAN) and 10 aa Histidine-based tag. It as a predicted molecular mass of 22.2 kDa, however as result of potential glycosylation, the recombinant protein co |
| AA Sequence : |
HHHHHHHHHHVPPGEDSKDVAAPHR QPLTSSERIDKQIRYILDGISALRK ETCNKSNMCESSKEALAENNLNLPK MAEKDGCFQSGFNEETCLVKIITGL LEFEVYLEYLQNRFESSEEQARAVQ MSTKVLIQFLQKKAKNLDAITTPDP TTNASLLTKLQAQNQWLQDMTTHLI LRSFKEFLQSSLRALRQM |
| Endotoxin : |
< 0.04 eu ug protein (lal> |
| Purity : |
>97% by SDS-PAGE gel |
| Applications : |
Cell culture, Western Blot. |
| Storage : |
This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted rh Il-6 should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended. |
| Reconstitution : |
Lyophilized protein should be reconstituted in water to a concentration of 50-100 ng/μl. Optimal concentration should be determined for specific application and cell lines. Optimal reconstitution please follow batch Quality Control sheet instructions. |
