Description : |
Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells. |
Source : |
E. coli |
Species : |
Human |
Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : |
ED50 < 0.1 ng/mL, measured by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells, corresponding to a specific activity of > 1 × 10^7 units/mg. |
Molecular Mass : |
20.9 kDa, observed by reducing SDS-PAGE. |
Protein Length : |
185 |
AA Sequence : |
MPVPPGEDSKDVAAPHRQPLTSSER IDKQIRYILDGISALRKETCNKSNM CESSKEALAENNLNLPKMAEKDGCF QSGFNEETCLVKIITGLLEFEVYLE YLQNRFESSEEQARAVQMSTKVLIQ FLQKKAKNLDAITTPDPTTNASLLT KLQAQNQWLQDMTTHLILRSFKEFL QSSLRALRQM |
Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
Purity : |
> 95% by SDS-PAGE analysis. |
Storage : |
Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : |
Lyophilized after extensive dialysis against 1xPBS. |
Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |