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Active Recombinant Human Interleukin 17A

Cat.No. : IL17A-113H
Product Overview : Recombinant Human Interleukin 17A encoding the human IL-17A protein sequence (containing the signalpeptide sequence, and the mature IL-17A sequence) was expressed in modified human293 cells.
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Cat. No. : IL17A-113H
Description : Interleukin-17 (IL-17) is a 155 amino acid protein that is a disulfide linked, homodimeric,secreted glycoprotein with one N-linked glycosylation site. IL-17 is also referred to as IL-17A. Other members include IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 binds tothe cell surfacer receptor IL-17R. IL-17 is secreted by a subset of T cells called T-helper 17 (Th-17) cells. IL-23 is thought to mediate the production of IL-17 by Th-17 cells.
Source : Human 293 cells.
Amino Acid Sequence : GITIPRNPGCPNSEDKNFPRTVMVN LNIHNRNTNTNPKRSSDYYNRSTSP WNLHRNEDPERYPSVIWEAKCRHLG CINADGNVDYHMNSVPIQQEILVLR REPPHCPNSFRLEKILVSVGCTCVT PIVHHVA.
Molecular Mass : Under reducing conditions Apollo IL-17A hcx migrates as two bands at approximately 16and 22 kDa on SDS-PAGE due to post-translational modifications.
pI : Apollo IL-17A hcx has a predicted pI of 8.62.
Glycosylation : Apollo IL-17A hcx contains N--linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-termstorage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Activity : The activity of IL-17A is measured by its ability to induce IL-6 production in NHDF cells. The ED50is typically between 0.5 and 1.5 ng/ml.
Gene Name : 17A interleukin 17A [ Homo sapiens ]
Synonyms : IL17A; interleukin 17A; IL17; CTLA8; IL-17; IL-17A; toxic T-lymphocyte-associated antigen 8; otoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
Gene ID : 3605
mRNA Refseq : NM_002190
Protein Refseq : NP_002181
UniProt ID : Q16552
Chromosome Location : 6p12
MIM : 603149
Pathway : Cytokine-cytokine receptor interaction
Function : cytokine activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Could you explain the role of IL17A in autoimmune disease progression using animal models? 05/22/2022

Animal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.

Can you detail the protocols for inducing IL17A expression in T cells in vitro? 01/02/2021

Inducing IL17A expression in T cells involves culturing them with specific cytokines.

What strategies elucidate IL17A's impact on tissue-specific inflammation in human samples? 02/17/2020

Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.

Can you elaborate on the use of neutralizing antibodies in blocking IL17A's effects? 06/12/2019

Neutralizing antibodies block IL17A's effects in cell cultures or animal models.

How does one perform a multiplex cytokine assay to quantify IL17A along with other cytokines? 12/13/2018

Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.

How is the IL17A receptor (IL17RA) signaling pathway activated and studied? 11/23/2018

IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.

What are the methods to investigate the impact of IL17A on epithelial barrier integrity? 08/09/2018

Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.

How is IL17A's contribution to neutrophil recruitment assessed in inflammatory models? 06/25/2018

Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.

What techniques are used to analyze IL17A-producing cell populations within a tissue? 06/22/2018

Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.

What experimental design is employed to understand IL17A's involvement in cancer development? 03/23/2018

Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.

Customer Reviews (3)

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Reviews
12/16/2018

    Good for receptor studies.

    03/05/2018

      Effective in Th17 differentiation.

      03/08/2017

        High bioactivity in various tissue types.

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