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Active Recombinant Human ITGA4 & ITGB1 Heterodimer Protein, His-tagged

Cat.No. : ITGA4&ITGB1-356H
Product Overview : Recombinant Human ITGA4 & ITGB1 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)). Predicted N-terminus: Tyr 34 (ITGA4) & Gln 21 (ITGB1). Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Tyr 34 (ITGA4) & Gln 21 (ITGB1). The reducing (R) protein migrates as 60-70 kDa and 80-85 kDa (ITGA4) and 100-133 kDa and 135-150 kDa (ITGB1) respectively due to glycosylation.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Recombinant Human ITGA4 & ITGB1 Heterodimer Protein, His-tagged with a linear range of 1-16 ng/mL (QC tested).
Molecular Mass : 111.4 kDa (ITGA4) & 83.7 kDa (ITGB1)
Protein length : Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) [ Homo sapiens ] & ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
Official Symbol : ITGA4 & ITGB1
Synonyms : ITGA4; CD49D; integrin alpha-4; CD49d; 269C wild type; IA4; MGC90518; & ITGB1; FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; FNRB; MDF2; VLAB; MSK12; VLA-BETA;
Gene ID : 3676 & 3688
mRNA Refseq : NM_000885 & NM_002211
Protein Refseq : NP_000876 & NP_002202
MIM : 192975 & 135630
UniProt ID : P13612 & P05556

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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