Active Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged
Cat.No. : | ITGAV&ITGB8-366H |
Product Overview : | Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201 (ITGAV) & NP_002205 (ITGB8)). Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8). Human ITGAV & ITGB8 Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.0 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120-130 kDa and 140-150 kDa (ITGAV) and 90-95 kDa (ITGB8) respectively due to glycosylation. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM MgCl2, pH8.0. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged at 5 μg/mL (100 μL/well) can bind Biotinylated Human TGF-Beta 1, His Tag, Avi Tag with a linear range of 0.8-13 ng/mL (QC tested). Immobilized Human Fibronectin at 10 μg/mL (100 μL/well) can bind Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged with a linear range of 0.313-10 μg/mL (Routinely tested). Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged with a linear range of 0.313-10 μg/mL (Routinely tested). |
Molecular Mass : | 113.0 kDa (ITGAV) & 76.5 kDa (ITGB8) |
Protein length : | Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | ITGAV integrin, alpha V [ Homo sapiens ] & ITGB8 integrin, beta 8 [ Homo sapiens ] |
Official Symbol : | ITGAV & ITGB8 |
Synonyms : | ITGAV; integrin, alpha V; CD51; MSK8; VNRA; VTNR; DKFZp686A08142; & ITGB8; integrin, beta 8; integrin beta-8; |
Gene ID : | 3685 & 3696 |
mRNA Refseq : | NM_001144999 & NM_002214 |
Protein Refseq : | NP_001138471 & NP_002205 |
MIM : | 193210 & 604160 |
UniProt ID : | P06756 & P26012 |
Products Types
◆ Recombinant Protein | ||
ITGAV&ITGB8-367H | Active Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His & Avi-tagged, Biotinylated | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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