Active Recombinant Human JAG1 protein, Fc-tagged

• Cat.No.: JAG1-379H
• Product Overview: Recombinant Human JAG1(Ser32-Ser1046) fused with Fc region of Human IgG1(Pro100-Lys330) tag at C-terminal was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ser32
• Form: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
• Bio-activity: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-2.0 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 137 kDa;SDS-PAGE: 170-180 kDa, under reducing conditions.
• Protein length: Ser32-Ser1046
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: JAG1 jagged 1 [ Homo sapiens ]
• Official Symbol: JAG1
• Synonyms: JAG1; jagged 1; AGS, Alagille syndrome , JAGL1; protein jagged-1; AHD; AWS; CD339; HJ1; AGS; JAGL1; MGC104644
• Gene ID: 182
• mRNA Refseq: NM_000214
• Protein Refseq: NP_000205
• UniProt ID: P78504
• Chromosome Location: 20p12.1-p11.23
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, conserved biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem
• Function: Notch binding; calcium ion binding; growth factor activity; structural molecule activity