Active Recombinant Human KITLG Protein

• Cat.No.: KITLG-961H
• Product Overview: Recombinant Human KITLG(Glu26-Ala189), transcript variant b, was expressed in E. coli.

Specification

• Description: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
• Source: E. coli
• Form: 1 x PBS
• Bio-activity: The ED50 is 3 -12 ng/ml, corresponding to a specific activity of 0.8 - 3.3 x 10^5 units/mg, determined by TF-1 cell proliferation induced in a dose dependent manner.
• Molecular Mass: 18.6 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method.
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer

Gene Information

• Gene Name: KITLG KIT ligand [ Homo sapiens ]
• Official Symbol: KITLG
• Synonyms: KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250
• Gene ID: 4254
• mRNA Refseq: NM_000899
• Protein Refseq: NP_000890
• MIM: 184745
• UniProt ID: P21583