Active Recombinant Human MDH2 Protein, His-tagged

• Cat.No.: MDH2-4499H
• Product Overview: Human MDH2 (NP_005909, 25 a.a. - 338 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.

Specification

• Description: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25 centigrade.
• Molecular Mass: 35.2 kDa
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMAKVA VLGASGGIGQPLSLLLKNSPLVSRL TLYDIAHTPGVAADLSHIETKAAVK GYLGPEQLPDCLKGCDVVVIPAGVP RKPGMTRDDLFNTNATIVATLTAAC AQHCPEAMICVIANPVNSTIPITAE VFKKHGVYNPNKIFGVTTLDIVRAN TFVAELKGLDPARVNVPVIGGHAGK TIIPLISQCTPKVDFPQDQLTALTG RIQEAGTEVVKAKAGAGSATLSMAY AGARFVFSLVDAMNGKEGVVECSFV KSQETECTYFSTPLLLGKKGIEKNL GIGKVSSFEEKMISDAIPELKASIK KGEDFVKTLK
• Purity: > 95% by SDS-PAGE
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at 4 centigrade for 1-2 weeks. For long term storage store at -20 centigrade or -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Concentration: 1 mg/mL
• Storage Buffer: In 20 mM Tris-HCl, pH 7.5 (10% glycerol)

Gene Information

• Gene Name: MDH2 malate dehydrogenase 2, NAD (mitochondrial) [ Homo sapiens ]
• Official Symbol: MDH2
• Synonyms: MDH2; malate dehydrogenase 2, NAD (mitochondrial); malate dehydrogenase, mitochondrial; MDH; MOR1; M-MDH; MGC:3559
• Gene ID: 4191
• mRNA Refseq: NM_005918
• Protein Refseq: NP_005909
• MIM: 154100
• UniProt ID: P40926