| Description : |
Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS |
| Bio-activity : |
The ED (50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL. |
| Molecular Mass : |
14.0 kDa |
| Endotoxin : |
<1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Purity : |
95% |
| Applications : |
Cell Assay |
| Storage : |
The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade- 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
Reconstitute at 0.1 mg/ml in sterile PBS |
