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Recombinant Human ME2 protein

Cat.No. : ME2-1274H
Product Overview : Recombinant Human ME2 protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
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Description : NAD-dependent malic enzyme (ME2), mitochondrial is a protein that in humans is encoded by the ME2 gene. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, which catalyzes the oxidative decarboxylation of malate to pyruvate. Three different isoforms of ME are known to be in mammalian tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent mitochondriail isoform, and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+. The mammalian isoforms size is about 62-64 kDa. A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM.
Molecular Mass : Approximately 64.4 kDa, a single non-glycosylated polypeptide chain containing 573 amino acids with 6 × His at C-terminus.
Protein length : 573
AA Sequence : MLHIKEKGKPLMLNPRTNKGMAFTL QERQMLGLQGLLPPKIETQDIQALR FHRNLKKMTSPLEKYIYIMGIQERN EKLFYRILQDDIESLMPIVYTPTVG LACSQYGHIFRRPKGLFISISDRGH VRSIVDNWPENHVKAVVVTDGERIL GLGDLGVYGMGIPVGKLCLYTACAG IRPDRCLPVCIDVGTDNIALLKDPF YMGLYQKRDRTQQYDDLIDEFMKAI TDRYGRNTLIQFEDFGNHNAFRFLR KYREKYCTFNDDIQGTAAVALAGLL AAQKVISKPISEHKILFLGAGEAAL GIANLIVMSMVENGLSEQEAQKKIW MFDKYGLLVKGRKAKIDSYQEPFTH SAPESIPDTFEDAVNILKPSTIIGV AGAGRLFTPDVIRAMASINERPVIF ALSNPTAQAECTAEEAYTLTEGRCL FASGSPFGPVKLTDGRVFTPGQGNN VYIFPGVALAVILCNTRHISDSVFL EAAKALTSQLTDEELAQGRLYPPLA NIQEVSINIAIKVTEYLYANKMAFR YPEPEDKAKYVKERTWRSEYDSLLP DVYEWPESASSPPVITEHHHHHH
Endotoxin : Less than 1 EU/μg of rHuME2, His as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : ME2
Official Symbol : ME2
Synonyms : ME2; malic enzyme 2, NAD(+)-dependent, mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; malate dehydrogenase; pyruvic-malic carboxylase; ODS1;
Gene ID : 4200
mRNA Refseq : NM_001168335
Protein Refseq : NP_001161807
MIM : 154270
UniProt ID : P23368

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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